Coccidioides Quantitative Antigen EIA

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  • Test Code

    315

  • Clinical Significance

    The MVista® Coccidioides quantitative EIA test aids in the diagnosis of coccidioidomycosis (especially in seronegative cases).  Monitoring the Coccidioides antigen helps in some cases to determine when treatment can be stopped and to diagnose relapse.

  • Methodology
    • – Quantitative Sandwich Enzyme Immunoassay (EIA)
    • – Serum and CSF are pre-treated to improve accuracy
  • Limitations

    The result is not intended to be used as the sole means for clinical diagnosis or patient management decisions.  Clinical sensitivity for diagnosis in dogs with pathology proven coccidioidomycosis is low (<40%); therefore, antibody testing is preferred for initial screening. Cross-reactions are seen with histoplasmosis, blastomycosis, paracoccidioidomycosis.  Sputolysin, sodium hydroxide and potassium hydroxide treatment degrade the analyte detected in the assay.

  • Specimen Collection
    • Serum: Collect serum specimens in serum separator or red top tube. Allow blood to clot for 30 minutes, then centrifuge. Pipette serum into a plastic screw cap vial.
    • Plasma: Collect plasma specimens in an EDTA, heparin or sodium citrate tube. Centrifuge for 15 minutes and pipette plasma into a plastic screw cap vial.
    • Urine/CSF/BAL/Other Body Fluids: Submit urine, CSF, BAL and all other body fluids in a sterile screw cap container.
  • Minimum Specimen Requirements
    • Serum/Plasma: 1.2 mL
    • CSF: 0.8 mL
    • Urine/BAL/Other Body Fluids: 0.5 mL
  • Specimen Stability
    • Room Temperature: 2 days
    • Refrigerated: 14 days
    • Frozen: Indefinitely
  • Specimen Rejection
    • – If specimen is too viscous to pipette
    • – Tissue, Sputum, Bronchial Brushings, Stool, FNA, Biopsy, Tracheal or Bone Marrow Aspirate
    • – Stored in Transport Media, Fixative or Isolator Tubes
  • Transport Temperature

    Refrigerated/Frozen/Room Temperature

  • Shipping

    Ship next day service for Monday-Friday delivery

  • Turnaround Time

    Testing is performed Monday-Friday.

    • Urine/BAL: Same day
    • Serum/Plasma/CSF: 1 day

    *New positives may require confirmation

     

  • Reference Range

    None detected

  • Interpretative Information
    • Positive: 0.07 – 8.2 ng/mL
    • Positive Above the Limit of Quantification: Results greater than 8.2ng/mL fall outside the linear range of the assay. These results are positive, but not accurately quantifiable.
  • Additional Information

    MVista® is a registered trademark of MiraVista Diagnostics

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