Medical-Diagnostics

Aspergillus Antigen EIA

  • Test Information
  • Specimen Requirements
  • Shipping Information
  • Test Code +

    309

    Clinical Significance +

    The Aspergillus Antigen EIA test aids in the diagnosis of systemic aspergillosis in animals. This test has low sensitivity in dogs with sino-nasal aspergillosis; therefore, the test is not recommended for screening in those cases.

    Methodology +

    Platelia™ Aspergillus EIA immunoenzymatic sandwich microplate assay, cleared by the FDA for the detection of galactomannan in serum or BALF.

    Limitations +

    Cross Reactivity/False Positive:
    • Certain gluconate containing fluids (Vetivex pHLyte; NOT Plasmalyte or Norm-R)
    • Potentially penicillin antibiotics
    • High: Alternaria, Fusarium, Geotrichum, Paecilomyces
    • Low: Coccidioides, Cryptococcus, Blastomyces, Histoplasma

    Turnaround Time +

    Test Setup:  Monday – Friday
    Results released: 12 – 24 hours

    *New positives may require confirmation

    Reference Range +

    Negative

    Interpretative Information +

    Negative: <0.5

    *Results are reported as numeric values (Index) which are interpreted as positive or negative.

    Additional Information +

    Platelia™ is a registered trademark of BioRad.

  • Specimen Collection +

    Specimen: Urine, Serum, Plasma, CSF, BAL

    Anticoagulant: EDTA

    Container:
    Serum/Plasma: Submit serum and plasma in a separator or transfer tube.
    Urine/CSF/BAL/Other Body Fluids: Submit urine, CSF, BAL or any other body fluids in a leak-proof container.

    Minimum Specimen Requirements +

    Volume: 0.4 mL

    Specimen Stability +

    • CSF:
      • Room temperature: 30 days
      • Refrigerated: 30 days
      • Frozen: 60 days
    • All other specimen:
      • Room temperature: 48 hours
      • Refrigerated: 5 days
      • Frozen: 5 months

    Specimen Rejection +

    If specimen is too viscous to pipette.

  • Transport Temperature +

    Refrigerated/Frozen

    Shipping +

    Ship overnight or 2nd day with cold pack.

* New positives may require confirmation.

** Turnaround times are provided as ranges, and may vary depending on daily testing volumes and specimen type.

*** Test set up: Samples received by 10:30AM will have same-day test set up. Positive samples may require confirmation which may extend turnaround time. Serum & CSF specimens are pretreated to improve sensitivity which can add one day to turnaround time.