Beta-D-Glucan Antigen
- Test Information
- Specimen Requirements
- Shipping Information
Test Code
317
Clinical Significance
The Fungitell® assay measures the presence of (1→3)-β-D-Glucan in serum and CSF. Serum is the only specimen type cleared by the FDA for this assay. Performance characteristics of CSF have been validated by MiraVista Diagnostics.
Methodology
Colorimetric Fungitell® assay utilizing a (1→3)-β-D-Glucan -specific Limulus Amebocyte Lysate (LAL) reagent.
Limitations
Interfering Substance: Turbid or grossly discolored samples due to lipid, bilirubin, or hemolysis. Significantly elevated immunoglobulin concentrations.
Cross Reactivity/False Positive:- Hemodialysis
- Penicillin antibiotics
- Fractionated blood products (serum albumin and immunoglobulins)
- Specimens exposed to gauze or paper wipes
Turnaround Time
Results released: 12 – 24 hours
Reference Range
None detected
Interpretative Information
Negative: <60 pg/mL
Indeterminate: 60 pg/mL – 79 pg/mL
Positive: 80 pg/mL – >500 pg/mLAdditional Information
* Fungitell® is a registered trademark of Associates of Cape Cod Inc.
Specimen Collection
- Specimen: Serum
- Uncommon Specimen: CSF
Container:
- Serum: Submit serum in a separator or transfer tube..
- CSF: Submit CSF in a sterile leak-proof container.
Minimum Specimen Requirements
Volume: 0.25 mL
Specimen Stability
- Refrigerated: 15 days
- Frozen: 27 days
Specimen Rejection
Turbid or grossly discolored samples due to lipid, bilirubin, or hemolysis or significantly elevated immunoglobulin concentrations may be rejected due to interference.
Transport Temperature
Refrigerated/Frozen
Shipping
Ship overnight with a cold pack.
* New positives may require confirmation.
** Turnaround times are provided as ranges, and may vary depending on daily testing volumes and specimen type.
*** Test set up: Samples received by 10:30AM will have same-day test set up. Positive samples may require confirmation which may extend turnaround time. Serum & CSF specimens are pretreated to improve sensitivity which can add one day to turnaround time.