The presence of IgG to Coccidioides assists in the diagnosis of coccidioidomycosis in dogs. IgG may be detected in some healthy dogs from the endemic area as a result of sub-clinical infection within the last 2 years. Intermediate results (8-9.9 EU) typically reflect either rising or falling IgG, and retesting the patient in several weeks may be beneficial.
Semi-Quantitative Indirect Enzyme Immunoassay (EIA)
Cross-reactivity/False Positives: Low: Histoplasma, Blastomyces, and Coccidioides (true positives are considerably higher than cross-reactive results)
The reference range and other method performance specifications have not been established for this test in CSF. The test results should be integrated into the clinical context for interpretation.
Testing is performed Wednesday & Friday.
Results Released: 24 hours
Negative: <8.0 EU
Intermediate: 8.0 – 9.9 EU
Positive: 10.0 – ≥80.0 EU
Specimen: Serum, CSF*, Plasma*
*Uncommon specimen includes incomplete validation. A ’rare specimen’ note will appear on the final report.
Serum: Collect serum specimens in serum separator tube. Allow blood to clot for 30 minutes, then centrifuge. Send serum in serum separator tube or transfer tube.
CSF, Plasma: Sterile transport tube
Anticoagulant: Heparin, EDTA, NaCit
Volume: 0.25 mL
Stability: Room temperature = 28 days, Refrigerated = 6 months, Frozen = indefinite
Any specimen types other than serum, plasmacerebrospinal fluid or if specimen exceeds stated stability ranges.
Overnight or 2nd day – ambient or with cold pack.