Test Code: 906
Panel Components:

• 403 MVista® Histoplasma DNA, PCR
• 404 MVista® Blastomyces DNA, PCR
• 405 MVista® Coccidioides DNA, PCR
• 406 MVista® Pneumocystis canis DNA, PCR

Specimen:

• 0.5mL bronchoalveolar lavage (BAL) fluid or cavitary effusion.
• Uncommon specimen: 0.5 mL tracheal aspirate or bronchial wash.

Clinical Significance:

Multiplex PCR allows for the simultaneous detection of multiple fungal pathogens from a single sample. This is especially valuable in patients presenting with non-specific respiratory symptoms or community-acquired pneumonia, where fungal infections may mimic bacterial or viral causes. These fungi (Histoplasma, Blastomyces, Coccidioides, and Pneumocystis) are common causes of opportunistic infections, particularly in HIV-positive or immunocompromised individuals. Early and accurate identification can significantly improve outcomes. In addition, patients may have co-infections with more than one fungal pathogen and multiplex PCR can identify these cases, allowing for more comprehensive treatment planning.