Coccidioides Antigen EIA

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The MVista® Coccidioides Quantitative EIA test aids in the diagnosis of coccidioidomycosis (especially in seronegative cases).  Monitoring the Coccidioides antigen helps in some cases to determine when treatment can be stopped and to diagnose relapse.

• Quantitative Sandwich Enzyme Immunoassay (EIA)
• Serum and CSF are pre-treated to improve accuracy

The result is not intended to be used as the sole means for clinical diagnosis or patient management decisions.  Clinical sensitivity for diagnosis in dogs with pathology proven coccidioidomycosis is low (<40%); therefore, antibody testing is preferred for initial screening. Sputolysin, sodium hydroxide and potassium hydroxide treatment degrade the analyte detected in the assay.

• Very High: Blastomyces, Histoplasma, Paracoccidioides and Talaromyces (talaromycosis, previously penicilliosis)
• Moderate: Coccidioides
• Rare: Aspergillus
• None: Cryptococcus

Testing is performed Monday – Friday.

Results released: 24 – 48 hours

*New positives may require confirmation

None detected

Positive: 0.07 – 8.2 ng/mL
Positive Above the Limit of Quantification: Results greater than 8.2 ng/mL fall outside the linear range of the assay. These results are positive, but not accurately quantifiable.

MVista® is a registered trademark of MiraVista Diagnostics

Specimen: Urine, Serum, Plasma, CSF, BAL

Serum: Collect serum specimens in serum separator tube. Allow blood to clot for 30 minutes, then centrifuge. Send serum in serum separator tube or transfer tube
Plasma: Collect plasma specimens in an EDTA, heparin or sodium citrate tube. Centrifuge for 15 minutes and pipette plasma into a plastic screw cap vial.
Urine/CSF/BAL/Other Body Fluids: Submit urine, CSF, BAL and all other body fluids in a leak-proof container.

Volume: Serum/plasma 1.2 mL, CSF 0.8 mL, Urine and BAL 0.5 mL
Stability: Room Temperature=2 weeks, Refrigerated=2 weeks, Frozen=indefinite

• If specimen is too viscous to pipette
• Tissue, Sputum, Bronchial Brushings, Stool, FNA, Biopsy, Tracheal or Bone Marrow Aspirate
• Stored in Transport Media, Fixative or Isolator Tubes

Refrigerated/Frozen/Room Temperature

Overnight or 2nd day – ambient.