Medical-Diagnostics
Medical-Diagnostics

DIAGNOSTIC TESTS

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Coccidioides Antigen EIA

  • Test Information
  • Specimen Requirements
  • Shipping Information

  • Test Code +

    315

    Clinical Significance +

    The MVista® Coccidioides Quantitative EIA test aids in the diagnosis of coccidioidomycosis (especially in seronegative cases).  Monitoring the Coccidioides antigen helps in some cases to determine when treatment can be stopped and to diagnose relapse.

    Methodology +

    • Quantitative Sandwich Enzyme Immunoassay (EIA)
    • Serum and CSF are pre-treated to improve accuracy

    Limitations +

    The result is not intended to be used as the sole means for clinical diagnosis or patient management decisions.  Clinical sensitivity for diagnosis in dogs with pathology proven coccidioidomycosis is low (<40%); therefore, antibody testing is preferred for initial screening. Sputolysin, sodium hydroxide and potassium hydroxide treatment degrade the analyte detected in the assay.

    Cross Reactivity/False Positive:
    • Very High: Blastomyces, Histoplasma, Paracoccidioides and Talaromyces (talaromycosis, previously penicilliosis)
    • Moderate: Coccidioides
    • Rare: Aspergillus
    • None: Cryptococcus

    Turnaround Time +

    Testing is performed Monday – Friday.

    Results released: 24 – 48 hours

    *New positives may require confirmation

    Reference Range +

    None detected

    Interpretative Information +

    Positive: 0.07 – 8.2 ng/mL
    Positive Above the Limit of Quantification: Results greater than 8.2 ng/mL fall outside the linear range of the assay. These results are positive, but not accurately quantifiable.

    Additional Information +

    MVista® is a registered trademark of MiraVista Diagnostics

  • Specimen Collection +

    Specimen: Urine, Serum, Plasma, CSF, BAL

    Serum: Collect serum specimens in serum separator tube. Allow blood to clot for 30 minutes, then centrifuge. Send serum in serum separator tube or transfer tube
    Plasma: Collect plasma specimens in an EDTA, heparin or sodium citrate tube. Centrifuge for 15 minutes and pipette plasma into a plastic screw cap vial.
    Urine/CSF/BAL/Other Body Fluids: Submit urine, CSF, BAL and all other body fluids in a leak-proof container.

    Volume: Serum/plasma 1.2 mL, CSF 0.8 mL, Urine and BAL 0.5 mL
    Stability: Room Temperature=2 weeks, Refrigerated=2 weeks, Frozen=indefinite

    Specimen Rejection +

    • If specimen is too viscous to pipette
    • Tissue, Sputum, Bronchial Brushings, Stool, FNA, Biopsy, Tracheal or Bone Marrow Aspirate
    • Stored in Transport Media, Fixative or Isolator Tubes

  • Transport Temperature +

    Refrigerated/Frozen/Room Temperature

    Shipping +

    Overnight or 2nd day – ambient.

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