Coccidioides Antigen EIA
- Test Information
- Specimen Requirements
- Shipping Information
Test Code
315
Clinical Significance
The MVista® Coccidioides Quantitative EIA test aids in the diagnosis of coccidioidomycosis (especially in seronegative cases). Monitoring the Coccidioides antigen helps in some cases to determine when treatment can be stopped and to diagnose relapse.
Methodology
- Quantitative Sandwich Enzyme Immunoassay (EIA)
- Serum and CSF are pre-treated to improve accuracy
Limitations
The result is not intended to be used as the sole means for clinical diagnosis or patient management decisions. Clinical sensitivity for diagnosis in dogs with pathology proven coccidioidomycosis is low (<40%); therefore, antibody testing is preferred for initial screening. Sputolysin, sodium hydroxide and potassium hydroxide treatment degrade the analyte detected in the assay.
Cross Reactivity/False Positive:- Very High: Blastomyces, Histoplasma, Paracoccidioides and Talaromyces (talaromycosis, previously penicilliosis)
- Rare: Aspergillus
- None: Cryptococcus
Turnaround Time
Testing is performed Monday – Friday.
Results released: 12 – 24 hours
*New positives may require confirmation
Reference Range
None detected
Interpretative Information
Positive: 0.07 – 8.2 ng/mL
Positive Above the Limit of Quantification: Results greater than 8.2 ng/mL fall outside the linear range of the assay. These results are positive, but not accurately quantifiable.Additional Information
MVista® is a registered trademark of MiraVista Diagnostics
Specimen Collection
Specimen: Urine, Serum, Plasma, CSF, BAL
Anticoagulant: Heparin, EDTA, NaCit
Container:
Serum/Plasma: Submit serum and plasma in a separator or transfer tube.
Urine/CSF/BAL/Other Body Fluids: Submit urine, CSF, BAL or any other body fluids in a leak-proof container.Minimum Specimen Requirements
Volume: 0.8 mL
Specimen Stability
- Room Temperature: 28 days
- Refrigerated: 28 days
- Frozen: 60 days
Specimen Rejection
- If specimen is too viscous to pipette
- Tissue, Sputum, Bronchial Brushings, Stool, FNA, Biopsy, Tracheal or Bone Marrow Aspirate
- Stored in Transport Media, Fixative or Isolator Tubes
Transport Temperature
Room Temperature/Refrigerated/Frozen
Shipping
Overnight or 2nd day – ambient.
* New positives may require confirmation.
** Turnaround times are provided as ranges, and may vary depending on daily testing volumes and specimen type.
*** Test set up: Samples received by 10:30AM will have same-day test set up. Positive samples may require confirmation which may extend turnaround time. Serum & CSF specimens are pretreated to improve sensitivity which can add one day to turnaround time.