Blastomyces Quantitative Antigen EIA

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  • Test Code


  • Clinical Significance

    The MVista® Blastomyces quantitative antigen EIA aids in the diagnosis of blastomycosis. Monitoring the Blastomyces antigen also assists in therapeutic decision-making and identification of relapses.

  • Methodology
    • – Quantitative Sandwich Enzyme Immunoassay (EIA)
    • – Serum and CSF are pre-treated to improve accuracy
  • Limitations

    The result is not intended to be used as the sole means for clinical diagnosis or patient management decisions.

    • – Cross-reactions are seen with histoplasmosis, paracoccidioidomycosis, penicilliosis, less frequently in coccidioidomycosis, rarely in aspergillosis and possibly sporotrichosis.
    • – Sputolysin, sodium hydroxide and potassium hydroxide treatment degrade the analyte detected in the assay.
  • Specimen Collection
    • Serum: Collect serum specimens in serum separator or red top tube. Allow blood to clot for 30 minutes, then centrifuge. Pipette serum into a plastic screw cap vial.
    • Plasma: Collect plasma specimens in an EDTA, heparin or sodium citrate tube. Centrifuge for 15 minutes and pipette plasma into a plastic screw cap vial.
    • Urine/CSF/BAL/Other Body Fluids: Submit urine, CSF, BAL and all other body fluids in a sterile screw cap container. Urine may be collected by cystocentesis or free catch in a sterile container.  Avoid collection of urine from the floor or cage enclosure.
  • Minimum Specimen Requirements
    • Serum/Plasma: 1.2 mL
    • CSF: 0.8 mL
    • Urine/CSF/BAL/Other Body Fluids: 0.5 mL
  • Specimen Stability
    • Room Temperature: 2 weeks
    • Refrigerated: 2 weeks
    • Frozen: Indefinitely
  • Specimen Rejection
    • – If specimen is too viscous to pipette
    • – Tissue, Sputum, Bronchial Brushings, Stool, FNA, Biopsy, Tracheal or Bone Marrow Aspirate
    • – Stored in Transport Media, Fixative or Isolator Tubes
  • Transport Temprature

    Refrigerated/Frozen/Room Temperature

  • Shipping

    Ship next day service for Monday-Friday delivery

  • Turnaround Time

    Testing is performed Monday-Friday.

    • Urine/BAL: Same day
    • Serum/Plasma/CSF: 1 day

    *New positives may require confirmation


  • Reference Range

    None detected

  • Interpretative Information
    • Positive Below the Limit of Quantification: Results above the cutoff for positivity but below 0.2 ng/mL fall outside the linear range of the assay. These results are positive, but not accurately quantifiable. Result will be confirmed and reported the next business day.
    • Positive: 0.2 – 14.7 ng/mL
    • Positive Above the Limit of Quantification: Results greater than 14.7ng/mL fall outside the linear range of the assay. These results are positive, but not accurately quantifiable.
  • Additional Information

    MVista® is a registered trademark of MiraVista Diagnostics

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