Coccidioides Antibody Immunodiffusion

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The Coccidioides antibody immunodiffsion test is used for the in vitro determination of precipitating antibodies to Coccidioides TP and F antigens. It is used as an aid in the diagnosis of Coccidioidomycosis.

Fungal Immunodiffusion

The result is not intended to be used as the sole means for clinical diagnosis or patient management decisions.

• – Negative serologic tests may be observed among culturally demonstrable cases, which limits the predictive value of a negative test.
• – Negative serologic results obtained during the first month may warrant repeat testing.
• – Falsely negative results may be obtained when testing immunocompromised patients.
• – Test results cannot be used solely to distinguish between active infection and prior exposure.

Serum: Collect serum specimens in serum separator or red top tube. Allow blood to clot for 30 minutes, then centrifuge. Pipette serum into a plastic screw cap vial.

Serum: 0.25 mL per antibody

Positive samples will automatically reflex to a titer.

• Refrigerated: 14 days
• Frozen: 6 months

Any specimen types other than serum.

Refrigerated/Frozen

Ship on dry ice or frozen packs for next day service. Monday-Friday delivery.

Testing is performed on Tuesdays and Fridays.

Serum: 3 days from setup

• – Antibody not detected
• – Will report F and TP specific

• Negative: Antibody not detected
• Positive: Antibody detected, will report titer up to ≥1:128

• – Antibodies to the TP antigen are typically detectable within 4 weeks of primary onset of infection but are rarely detected 6 months after infection. The presence of antibodies to the TP antigen may suggest recent or active infection.
• – Antibodies to the F antigen are typically detectable by 6 weeks after the primary onset of the infection and may persist for several months to years.